5 Key Takeaways

• 1

  Phenylacetylglutamine (PAGIn) has been misidentified as phenylacetylglycine (PAGly) in nearly half of NMR studies on human samples.

• 2

  The misidentification of PAG and PAGly has persisted despite correct assignments being known for decades.

• 3

  Errors in metabolite identification are widespread due to reliance on spectral databases and careless practices.

• 4

  Correct metabolite assignment is crucial for understanding biological pathways and ensuring accurate biomedical interpretations.

• 5

  The metabolomics community must implement rigorous validation processes to prevent similar misidentifications in the future.