Upcoming Webinars for 2018

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Assessing Critical Quality Attributes and Structural Conformation of Biotherapeutics by HDX-MS

Hydrogen deuterium exchange (HDX) mass spectrometry (MS) is a powerful tool for studying the dynamics of higher order structure of protein-based therapeutics. Learn from experts at Genentech and Thermo Fisher Scientific how HDX-MS can be employed to monitor critical quality attributes of protein therapeutics.

Learning Objectives of Webinar

  1. Learn how HDX-MS can provide a high resolution structural comparison of biotherapeutics with high sensitivity and speed.
  2. Learn how the potential quality impact of product variants can be assessed through various tools including HDX-MS.
  3. Learn how HDX-MS can be used to get structural insights into the higher order conformation of therapeutic proteinsLearn how electron transfer dissociation for HDX-MS top-down & bottom up methods are developed and see how multi fragmentation methods support protein sequence coverage.
  4. See how those methods were successfully applied to study apo-, holo- calmodulin conformation forms.

25th September 2018, 8.30 am PDT, 11.30am EDT, 17.30pm CEST

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Accurate peptide retention data collection for retention modeling studies using the Q Exactive HF-X Orbitrap mass spectrometer

Introduction of proteomics provided separation scientists with virtually unlimited source of retention data: identification and assignment of retention properties of tens of thousands peptides in a few hours of instrument time has become a routine task. Nevertheless, accurate retention data collection for retention modeling or construction of comprehensive retention libraries for data independent acquisition protocols still represents a formidable task. This presentation provides step-by-step guide for construction of peptide retention library using 2D LC-MS/MS (high pH RP – low pH RP separation scheme, Q Exactive HF-X Orbitrap mass spectrometer) analysis of whole cell tryptic digests.

Learning Objectives of Webinar

  1. To introduce the concept of “chromatographic quality control” for accurate retention data collection via application of peptide retention standards;
  2. To learn about major parameters of separation system affecting separation selectivity, i.e. accuracy of retention data collection;
  3. To estimate expected output from a typical 2D LC-MS/MS acquisition using Q Exactive HF-X platform.

27th September 2018, 16:00 BST

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Top-Down characterization of a site-specific antibody-drug conjugate

In this webinar, we show how rapid high sequence coverage of mAbs and ADCs can be achieved with advanced top-down fragmentation capabilities such as Higher-energy Collision Dissociation (HCD), Electron Transfer Dissociation (ETD) and Ultra-Violet Photo-Dissociation (UVPD). In addition, we show that the technique can pinpoint sites of oxidation resulting from oxidative stress. Finally, learn about advances in automated software processing that easily map the protein structure and provide confirmation of sequence coverage and process induced modifications.

Join experts from University of Strasbourg and Thermo Fisher Scientific to learn about the latest technology, software and research applications for this highly promising technique.

Learning Objectives of Webinar

  1. Learn how high top or middle-down sequence coverage can obtained by combining multiple modes of fragmentation.
  2. Learn about new innovations in mass spectrometry and data processing software for top or middle-down characterisation.
  3. Learn how middle-down experiments could be a valuable alternative assay to peptide mapping for fast amino acid sequence confirmation.

31st October 2018, 9am PDT, 12pm EDT, 5pm CET

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Intact Analysis and Mapping of Therapeutic Protein Variants using Ion Exchange Chromatography Mass Spectrometry, and Peptide Sequencing via Fragmentation

Join experts from leading contract research organisation, Covance, and learn how they have developed capabilities for the analysis of antibody-drug conjugate variants using ion exchange chromatography hyphenated to MS. Also hear how Covance are able to confirm primary peptide sequence of intact peptides, without enzymatic digestion, using MS fragmentation.

Learning Objectives of Webinar

  1. Learn about optimization of charge variant separations of ADCs for analysis by mass spectrometry.
  2. Learn peptide sequencing by fragmentation, how to optimize fragmentation parameters, and data processing conditions.
  3. Learn about the latest technology for advanced therapeutic protein characterization.

5th December 2018, 9am PST, 12pm EST, 6pm CET

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