Analysis of the Vitamin B Complex in Infant Formula Samples

contributed by SCIEX | 07/14/2014

A rapid, robust, sensitive and specific LC-MS/MS assay using the AB SCIEX QTRAP^® 6500 system has been developed for the simultaneous detection of all major forms of vitamin B complex. The method detects all currently used forms of vitamin B6 and vitamin B3 in infant formula and includes vitamin B12. The sample preparation allows the same extract to be used for Vitamin C detection and the LC-MS/MS conditions have been tuned so that the response for each vitamin is linear over the various required detection ranges.

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