Objective:

To review the application of Raman spectroscopy and its enhanced variants in diagnosing pandemic-prone viruses, emphasizing its significance in pandemic preparedness.

Key Findings:

• Raman-based methods enable rapid detection of influenza and SARS-CoV-2 with high accuracy.
• Multiplexed detection of viruses in complex samples is feasible.
• Research focus is skewed towards accessible viruses, leaving high-risk pathogens like Lassa and Marburg underexplored.

Interpretation:

While Raman spectroscopy shows promise for rapid viral diagnostics, significant technical and regulatory challenges hinder its clinical application, necessitating focused efforts to address these issues.

Limitations:

• Weak signal intensity and fluorescence interference limit detection capabilities.
• Lack of standardization affects reproducibility.
• Biosafety constraints restrict research on high-risk pathogens, highlighting the need for improved measures.

Conclusion:

Raman spectroscopy has potential in pandemic preparedness, but overcoming technical barriers and regulatory challenges is essential for real-world diagnostics, which could significantly impact public health.