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Techniques & Tools Liquid Chromatography, Technology, Proteomics, Genomics & DNA Analysis, Gas Chromatography, Mass Spectrometry

Marking Progress

What is the current state of play?

Monika Dittman: In 1D-HPLC, using sub-2µm particles and (U)HPLC instruments operating at over 1000 bar, the time to reach a desired plate number has been reduced roughly by a factor of 5 (30,000 plates in four minutes with 1.8 µm particles at 1000 bar compared to 20 minutes with 5 µm particles at 300 bar) in routine analysis. The gain in performance can be used to obtain higher resolution in the same separation time (typically a factor of 3–4 on plate number or a factor of 1.5 in resolution/peak capacity), depending on the separation conditions.

These gains have enabled the fast separation of complex samples, and reduced the effort and time required for method development because a lack in selectivity is counteracted by increasing efficiency.

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About the Authors

Monika Dittman

In 1988, after finishing her PhD and spending two years as a post-doc at UC Berkeley, USA, Monika Dittmann joined HP (now Agilent Technologies) as an R&D scientist. In her career with HP/Agilent she has worked on the development of instruments and technologies in the fields of HPLC, Capillary Electrophoresis, Capillary Electro-Chromatography and Micro Fluidics as a research scientist and project manager. Monika currently holds the position of a Principal Scientist working on the design and development of next generation HPLC systems.


Fabrice Gritti

Fabrice Gritti is based at the Waters Corporation, Milford, USA.

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