Monika Dittman (Agilent Technologies) and Fabrice Gritti (Waters) offer a manufacturer point of view on the practical limits of LC.
Monika Dittman, Fabrice Gritti |
What is the current state of play?
Monika Dittman: In 1D-HPLC, using sub-2µm particles and (U)HPLC instruments operating at over 1000 bar, the time to reach a desired plate number has been reduced roughly by a factor of 5 (30,000 plates in four minutes with 1.8 µm particles at 1000 bar compared to 20 minutes with 5 µm particles at 300 bar) in routine analysis. The gain in performance can be used to obtain higher resolution in the same separation time (typically a factor of 3–4 on plate number or a factor of 1.5 in resolution/peak capacity), depending on the separation conditions.
These gains have enabled the fast separation of complex samples, and reduced the effort and time required for method development because a lack in selectivity is counteracted by increasing efficiency.
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