Trace-level analysis of VOCs in a tomato product using headspace extraction with large volume preconcentration (LVP) and multi-step enrichment (MSE)

In this application note, we demonstrate the benefit of a novel technique – large volume preconcentration (LVP) – for enhancing traditional headspace analysis of trace-level volatile organic compounds (VOCs) that contribute to aroma and flavour in a food product – tomato paste. Using the Centri^® sample extraction and enrichment platform in headspace–trap mode, the extracted analytes are preconcentrated on an electricallycooled, cryogen-free focusing trap. This allows for large extraction volumes to be used (up to 5 mL) while maintaining optimum chromatographic performance. We also highlight the benefits of multi-step enrichment (MSE), whereby repeat extractions from a single vial or multiple vials are delivered to the trap prior to a single GC injection. This results in a further improvement in the discovery and confident identification of trace-level aroma and flavour compounds.