Biotage

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of acrylamide from coffee using ISOLUTE® SLE+ columns with LC-MS/MS detection.

This application note describes the extraction of a range of SPICE drugs and metabolites from neat oral fluid and oral fluid from a commercial collection kit using ISOLUTE® SLE+ in both 96-well plate and column formats.

This application note describes the extraction of the acidic catecholamine metabolites VMA, 5-HIAA, and HVA from synthetic urine using EVOLUTE® EXPRESS AX 96-well SPE plates.

The method described in this application note achieves high reproducible recoveries for a wide range of drugs of abuse and metabolites from hydrolyzed urine. Hydrolyzed urine was extracted using the ISOLUTE SLE+ 200 supported liquid extraction plate and 1 mL column formats using 100 and 500 μL sample volumes, respectively. Sub ng/mL levels were achieved for all analytes using 100 μL of urine on the fixed well plate format. ISOLUTE® SLE+ products provide clean, rapid, robust, efficient, high throughput and automatable extraction solutions for a wide range of analytes.

This application note describes a method of extraction for metanephrine and normetanephrine from synthetic urine using EVOLUTE EXPRESS CX 96 fixed well plates.

This application note describes a Solid Phase Extraction (SPE) protocol for the extraction of a range of mycotoxins from wheat flour, wheat, maize and barley using ISOLUTE® Myco with LC-MS/MS.