Techniques & Tools Mass Spectrometry, Data Analysis

Top Three Trends in Lipidomics

State-of-the-art lipidomic analysis is based on two mass spectrometric approaches used for quantitation: shotgun lipidomics (using electrospray ionization MS/MS) or LC-MS.

05/15/2015

Michal Holcapek, Professor University of Pardubice

State-of-the-art lipidomic analysis is based on two mass spectrometric approaches used for quantitation: shotgun lipidomics (using electrospray ionization MS/MS) or LC-MS. The shotgun approach provides fast quantitation, while LC-MS can provide more detailed information on isomers. Michal will discuss the potential of various chromatographic modes, including SFC, and also cover MS imaging – the third analytical platform used to determine spatial distribution of lipids and other biomolecules. Finally, Michal will introduce useful multivariate data analysis methods.

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