Cookies

Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.
Subscribe to Newsletter

CE-SDS analysis of Etanercept using BioPhase 8800 system

CE-SDS analysis of Etanercept using BioPhase 8800 system

Introduction
 

Etanercept has been widely used in the treatment of plaque psoriasis, psoriatic arthritis, ankylosing spondylitis, and rheumatoid arthritis for over 20 years.1, 2 It works as a tumor necrosis factor (TNF) antagonist by binding to TNF molecules and preventing them from binding to the cell-bound receptors—which could otherwise lead to autoimmune disease when de-regulated TNF production occurs.3 Etanercept is a heavily glycosylated fusion protein consisting of the extracellular ligand-binding domain of human TNF receptor 2 (TNFR2) and the Fc domain of human IgG1.4 There are 2 N-glycosylation sites and 13 potential O-glycosylation sites on the TNFR2 domain and one N-glycosylation site on the Fc domain.4 The glycosylation alone represents approximately 30% of the total molecular weight of etanercept.5 The fusion protein is also a homodimer where disulfide bonds connect the two monomers. These properties combined make the protein structure very complex and highly heterogeneous. The physicochemical characterization of such molecules is very challenging.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Login
Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Most Popular
Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register