Fields & Applications Pharma & Biopharma, Technology, Liquid Chromatography, Mass Spectrometry

Monoclonal Antibody Peptide Mapping Using Micro Pillar Array Columns Combined with Mass Spectrometry (μPAC™-MS) – Part 2

contributed by Pharma Fluidics | 10/09/2017

Remicade® originator and candidate biosimilar

The use of micro pillar array columns (μPAC™) for monoclonal antibody (mAb) peptide mapping is presented. Remicade® (iniximab) originator and candidate biosimilar tryptic digests were analyzed on a 200 cm μPAC™ C18 column and peaks eluting were detected by ultraviolet (UV) spectroscopy and high-resolution mass spectrometry (MS).

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