Online native HIC-MS analysis of cys-linked ADCs

contributed by YMC | 11/03/2020

For the more detailed determination of the drug-to-antibody ratio (DAR) of antibody-drug-conjugates (ADCs) a coupling of hydrophobic interaction chromatography (HIC) and mass spectrometry (MS) is highly desirable as this eliminates the intermediate isolation step. Due to high concentrations of non-volatile salts, which are usually used in HIC mode, a coupling to MS would seem impossible and the use of volatile salts requires an even higher concentration to achieve the same salting-out effect.

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