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Biotage

Application Notes by Biotage

Fields & Applications Environmental

Concentrating a Suite of Semi-Volatile Compounds from EPA Method 625 Using the New Biotage TurboVap® II

| Contributed by Biotage

There are a wide range of volatile and semi-volatile contaminants finding their way into both terrestrial bodies and water sources worldwide.

Fields & Applications Forensics

Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis

| Contributed by Biotage

This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization.

Fields & Applications Food, Beverage & Agriculture

Analysis of Acrylamide in Crisps using LC-MS/MS

| Contributed by Biotage

Acrylamide is a known carcinogen, formed by cooking foodstuffs at high temperatures.

Acid Herbicides in Water with On-line SPE-LC-MS/MS (AN863)

| Contributed by Biotage

This application note describes the use of ISOLUTE® ENV+ on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 16 acid herbicides in drinking and surface water.

Simultaneous Extraction of Catecholamines and Metanephrines from Plasma for Analysis using LC-MS/MS

| Contributed by Biotage

A highly sensitive, linear and rugged extraction procedure for Catecholamines and Metanephrines from human plasma is presented.

Analysis of Pharmaceuticals in Water Using Automated On-line SPE-LC-MS/MS

| Contributed by Biotage

This application note describes the use of EVOLUTE® EXPRESS ABN on-line cartridges for extraction of 13 commonly prescribed pharmaceuticals in water.

Methylmalonic Acid: Evaluation of Sample Preparation and Simplicity of Method Implementation

| Contributed by Biotage

This poster is a summary of various sample preparation strategies for the extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. Also, the simplicity of transfer to an automated sample preparation platform is evaluated.

Extraction of Urinary Hormone Metabolites using Supported Liquid Extraction prior to HPLC-MS/MS Analysis.

| Contributed by Biotage

Presented here is a rapid and reliable sample preparation method using Support Liquid Extraction (SLE) to extract a suite of 30 urinary steroid hormone metabolites. Matrix effects are eliminated by the ISOLUTE® SLE+ protocol giving a sensitive, linear assay with excellent clinical utility.

Buprenorphine and Norbuprenorphine Extraction from Whole blood Using ISOLUTE® SLE+ Before LC-MS/MS

| Contributed by Biotage

This application note describes the extraction of buprenorphine and norbuprenorphine from whole blood using ISOLUTE® SLE+ Supported Liquid Extraction prior to LC-MS/MS analysis.

Estrone and Estradiol Extraction from Human Serum Using ISOLUTE® SLE+ Before LC-MS/MS

| Contributed by Biotage

This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum.

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