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Supelco

Application Notes by Supelco

The Potential of SPE for Fractionation of Serum Metabolome into Phospholipids and Other Metabolites

| Contributed by Supelco

The high concentration of phospholipids (PLs) in blood serum is a frequent limitation in metabolomic analysis based on mass spectrometric detection, mainly owing to their ionization suppression effects.

Faster Protein and Peptide Liquid Chromatography (FP2LC)

| Contributed by Supelco

Larger porous shell particles with narrow particle size distribution - used in reversed-phase U/HPLC analysis of peptides and proteins - provide increased efficiency per unit pressure drop, which can be taken advantage of by increasing peak capacity or traded in to reduce analysis time.

Selective Extraction and Cleanup of Patulin Mycotoxin from Apple Juice Samples

| Contributed by Supelco

In this application, the use of SupelMIP SPE – Patulin is demonstrated for the cleanup of patulin in apple juice.

GC Analysis of Fatty Acids in Walnuts and Peanuts

| Contributed by Supelco

Nuts are considered nutrient dense foods and their consumption has been associated with reduced risk of coronary heart disease. The health benefits of nuts are partially attributable to their high content of unsaturated fatty acids.

Investigating Matrix Interference in Analysis of Antiarrhythmic Cardiac Drugs in Plasma

| Contributed by Supelco

In this study the impact of matrix interference is investigated with respect to precision and accuracy of antiarrhythmic cardiac drugs from plasma samples.

Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers

| Contributed by Supelco

Chiral 2-hydroxyglutarates (2-OHG) are important molecular signatures of their specific biochemical pathways and inborn errors of metabolism, for both healthy and diseased biological cells

Analysis of Isocyanates Using the ASSET™ EZ4-NCO Dry Sampler

| Contributed by Supelco

The highly reactive nature of the isocyanate compounds and the low occupational exposure limits put high demands on both sampling and analytical techniques for monitoring of isocyanates in air.

Comprehensive Determination of Trans Fats in Cookies

| Contributed by Supelco

Extraction of lipids as fatty acids and the subsequent detection of trans fats from a cookie sample, using Silver-Ion SPE fractionation followed by GC analysis on SP-2560 and SLB-IL111 columns

A Single-Column Application. For the GC Determination of Benzene in Reformulated Gasoline using a Supelco SLB-IL111 Ionic Liquid Column

| Contributed by Supelco

The amount of benzene in gasoline is a concern because it is a known human carcinogen, and exposure to it has been linked to detrimental health effects.

Analysis of Vitamin D Metabolites in Serum

| Contributed by Supelco

Improvement in LC-MS/MS Analysis by Leveraging Column Selectivity and Effective Sample Prep

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