Explore Targeted Omics with Agilent
Discover how targeted metabolomics and lipidomics can bring clarity to your biomarker research with a selection of articles, application notes, and webinars from Agilent
Right on Target: Daniel Cuthbertson Explains Why There’s a Buzz Around Targeted Metabolomics
“In targeted metabolomics, you are often conducting hypothesis-driven research – really drilling down to the biological pathways most likely impacted by the disease of interest. The value of a targeted approach is that it’s much easier for a researcher to implement and makes the data much clearer. This reduces not only sample costs, but also labor costs by allowing a more standardized data analysis pipeline that requires fewer hours to review and makes results easier to interpret.
“This ultimately allows targeted metabolomics methods to be adopted for small-scale experiments and then easily scaled up to large cohort studies for biomarker research…”
Click here to read the rest of the article.
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A Comprehensive, Curated, High‑Throughput Method for the Detailed Analysis of the Plasma Lipidome
The use of advanced lipidomic techniques such as liquid chromatography/tandem mass spectrometry (LC/MS/MS) to study the human lipidome at the population level has enabled researchers to probe the relationships between disease states and lipid metabolism. Such studies necessarily involve large sample numbers from population cohorts. Capturing a comprehensive picture of the lipidome requires the analysis of several hundred different lipid species, and thus a balance between depth of coverage and sample throughput. This application note describes a targeted LC/TQ-based method for the determination of 763 different human lipid species using a 10 μL volume of plasma. The total analysis time was 16 minutes per sample using a single column, reduced to 13 minutes when running a dual column system. Also discussed are considerations for sample preparation and lipid extraction, quality control strategies, internal standard formulation, instrument conditions, and data processing.
An End-to-End Targeted Metabolomics Workflow
Targeted metabolomics aims to routinely detect and quantify a predefined group of metabolites likely to be involved in biological processes of interest. This application note presents a robust workflow that combines sample preparation and instrumental analysis solutions for targeted metabolomics, and can be applied to plasma and mammalian cell sample types.
Webinar: A Comprehensive, Turnkey Targeted Method for Plasma Lipidome Profiling Across Large Cohorts
Webinar: Jump Starting Your Targeted Metabolomics Workflow: New Methods for Automated Sample Prep, HILIC Separation, and Comprehensive MRMDatabase
