Materials

Copolymer analysis can be complex. The 2D approach is the only way to determine molar mass and chemical composition distributions simultaneously.

In this application note, one protein, interferon α-5, was conjugated with two different PEG molecules in different reactions.  The protein and PEG molecules were characterized individually using multi-detector SEC, followed by the PEGylated conjugates.  Their composition, molecular weights and intrinsic viscosities were measured. This work was performed by Celares GMBH, Germany with samples provided by DIGNA BIOTECH, Spain

As in HPLC before there is now a trend in GPC/SEC to separate low molar mass polymers and oligomers on columns filled with small particles.

Raman analysis of graphene on silicon dioxide wafers

This application note presents an example of how calorimetry aided the formulation development for ProX by providing insight into excipient-protein interactions. Polysorbate-80 and phenol are examined here as potential additives to the formulation buffer for ProX.

The study of exosomes and other cell-derived microvesicles is an area of rapidly rowing importance and the subject of intense interest and research. The previous lack of suitable methods for their detection, analysis, enumeration and phenotyping is proving to be a significant limitation in these studies. This document shows the degree to which the technique of Nanoparticle Tracking Analysis (NTA) is helping to address these problems.

Analytical QbD (AQbD) promises to extend the rigor and benefits of QbD into the area of analytical method development.

New components and measurement techniques continue to be introduced for the Zetasizer Nano. New to the system are a Surface Zeta Potential accessory and a new diffusion barrier method for improved zeta potential measurements of protein samples. Why is the Malvern Zetasizer Nano so popular and found in a broad range of applied and fundamental scientifi c projects across all industries and fields? Because, it is simply the most dynamic and broad ranged instrument on the market for solving technical issues in today’s research environment and it continues to evolve with new capabilities. Here are 10 reasons why you should choose the Zetasizer Nano:

The Zetasizer NanoSampler is a sample delivery system that ensures highly precise and reproducible automated loading of samples into your Zetasizer Nano for particle size measurements. The NanoSampler adds automation and unattended operation in a versatile, compact package, maximising the productivity of your Zetasizer Nano.

Agilent Technology’s Markets and Applications Programs – MAPs for short – connect you to a growing network of expertise to provide working solutions for your analytical business.