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Techniques & Tools Mass Spectrometry

High-throughput and data-independent analysis

sponsored by SCIEX

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May 27, 2020, 2pm CEST

Mass spectrometry is becoming increasingly important in the field of life sciences because of its high applicability for the analysis of biological samples. In this webinar, we will focus on strategies for metabolite quantification and identification using a QQQ (SCIEX QTRAP® system) and a QTOF (SCIEX X500R System) mass spectrometer. We will demonstrate a solution on how to overcome matrix effect in a little amount of time and how to run 12000 injections without suffering from instrument downtime. On the other hand, we will explore the versatility of data independent analysis techniques in terms of metabolite identification and quantification. Fixed and variable SWATH® Acquisition will be compared with MSALL in terms of the complexity of the acquired MS/MS spectra and the presence of product ion overlap.

Learning Objectives

  • High throughput and robust analyses of 12000 injections
  • Selectivity of different data independent analysis strategies
  • Quantitative and qualitative profiling methods

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