Separation of structural analogues: Vitamin D2 and D3

Introduction

Vitamin D₂ (ergocalciferol) and D₃ (cholecalciferol) can be found in different foods including fatty fishes, meat, eggs and some mushrooms. Both are (indirectly) involved in a number of biological functions in the body, including bone metabolism and enhancement of intestinal absorption of calcium, iron, magnesium, phosphate and zinc. A regular intake of vitamin D therefore is essential.

Separations of structurally similar compounds such as vitamin D₂ and vitamin D₃ are very challenging.

Standard C18 columns such as YMC-Triart C18 are not able to fully separate the two vitamins (Rs = 0.87). A very hydrophobic phase with a higher density and therefore amount of C18 chains is required. YMC-Triart C18 ExRS is able to separate these two with a resolution of Rs = 1.59 under the same conditions.

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