Subscribe to Newsletter

Discover how Bristol-Myers Squibb uses electron-activated dissociation (EAD) to confidently identify drug metabolites and localize site of metabolism.

sponsored by SCIEX

Register now

During drug metabolite identification (Met ID) studies collision-induced dissociation (CID) is the most commonly used fragmentation technique for LC-MS experiments. Many conjugation metabolites are found to have multiple bonding potentials to parent drugs. It is a great challenge to locate the bonding sites by CID due to the information lost by highly selective cleavage on these bonds. 

The novel electron-activated dissociation (EAD) is a recently developed technology on the SCIEX ZenoTOF 7600 system. From a single LC-MS/MS experiment CID and EAD data can be collected, providing information rich fragmentation spectra. The additional information can help in confident localization and identification of metabolites compared to CID alone. These fragments can be crucial to locate the metabolic modification sites, such as conjugations. 

In this study, the application of EAD in drug metabolite profiling is explored. The efficiency in the structural elucidation of conjugation metabolites is compared with that of CID.

Webinar Learning Objectives:

  • Learn how EAD can be advantageous in confident identification and localization of drug metabolites
  • Discover the sensitivity of the Zeno trap technology in the identification of the low abundant metabolites
  • Explore the capabilities of the Molecule Profiler software in processing EAD and CID data for drug metabolite identification

Register now

Visit our Webinar Hub

Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Related Application Notes
Separation of plasmid isoforms using BioPro HIC BF

| Contributed by YMC

Enabling 3D Multiplexing Spatial Omics Workflows in Neuroscience

| Contributed by Zeiss

Safer AAV Analysis with Non-toxic AEX Method

| Contributed by Tosoh

Related Product Profiles
Higher Peaks – Clearly.

| Contributed by Shimadzu Europa

Compact with countless benefits

| Contributed by Shimadzu Europa

The fine Art of Method Development

| Contributed by Shimadzu Europa

Related Events

Leuven, Belgium

SPEC2024, 13th International Conference on Clinical Spectroscopy

Ioannina, Greece

52nd International Symposium on High Performance Liquid Phase Separations and Related Techniques

Denver, Colorado, USA

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine