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Biotage

Application Notes by Biotage

Ultra-Sensitive Method for the Determination of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 in Serum Using Supported Liquid Extraction Prior to LC-MS/MS

| Contributed by Biotage

This application describes the use of ISOLUTE SLE+ for ultra-sensitive determination of 1,25 di-OH Vitamins D2 and D3.

Acidic, Basic, and Neutral Drug Screen of Hydrolyzed Urine Using Supported Liquid Extraction Prior to LC-MS/MS Analysis

| Contributed by Biotage

This application note describes the extraction of acidic, basic, and neutral drugs from urine for screening purposes using ISOLUTE® SLE+ supported liquid extraction plates prior to LC-MS/MS analysis.

Extraction of Buprenorphine and Norbuprenorphine from Oral Fluid Using EVOLUTE® EXPRESS CX Prior to LC-MS/MS Analysis

| Contributed by Biotage

This application note describes a simple and robust mixedmode cation exchange solid phase extraction protocol

Extraction of Epinephrine, Norepinephrine and Dopamine from Human Plasma Using EVOLUTE® EXPRESS WCX Prior to LC-MS/MS Analysis

| Contributed by Biotage

This application note describes the use of EVOLUTE® EXPRESS WCX for extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma before LC-MS/MS.

Extraction of Anabolic Steroids from Horse Urine using ISOLUTE® SLE+ for LC-MS/MS

| Contributed by Biotage

This application note describes a Supported Liquid Extraction (SLE) method for extraction of ethylestrenol and stanozolol from both gelding and filly horse urine prior to LC-MS/MS analysis. The method from Biotage shows high reproducible analyte recoveries.

Extraction of monohydroxy and dihydroxy Vitamin D metabolites from serum

| Contributed by Biotage

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of both forms of 1,25 diOH Vitamin D metabolite and both forms of 25 OH metabolite in serum using ISOLUTE®SLE+ plates with LC-MS/MS detection.

Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE® PLD+ prior to LC-MS/MS Analysis

| Contributed by Biotage

This application note describes the extraction of 25-hydroxy vitamin D from serum, prior to LC-MS/MS analysis.

Extraction of Polyfluorinated Compounds in Water Using EVOLUTE® WAX Prior to LC-MS/MS Analysis

| Contributed by Biotage

This application note describes a Biotage polymer-based mixed-mode weak anion exchange SPE protocol for the extraction of a range of polyfluorinated (PFA) compounds in water prior to LC-MS/MS detection.

Comparison of Biotage® Extrahera™ vs. Manual Sample Processing Using a Vacuum Manifold

| Contributed by Biotage

Comparison of Biotage® Extrahera™ vs. Manual Sample Processing of vitamin D using ISOLUTE SLE+

Extraction of gamma-Hydroxybutyric Acid (GHB) from Urine Using ISOLUTE® SLE+ Prior to GC/MS Analysis

| Contributed by Biotage

This application note describes the extraction of GHB from urine using supported liquid extraction and subsequent analysis by GC/MS.

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