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Biotage

Application Notes by Biotage

Extraction of Aflatoxins and Ochratoxin from Dried Chili Using ISOLUTE® Myco Prior to LC-MS/MS Analysis

| Contributed by Biotage

An SPE protocol for the extraction of a range of mycotoxins from dried chili using ISOLUTE® Myco with LC-MS/MS analysis, achieving high recoveries of relevant mycotoxins with %RSDs and LOQs that meet EU regulations for measurement of these analytes.

Biomonitoring of Phthalate Metabolites in Human Urine using ISOLUTE® ENV+ Columns Prior to LC-MS/MS

| Contributed by Biotage

This application note describes the extraction of nine phthalate metabolites from human urine using ISOLUTE ENV+ solid phase extraction columns. This method demonstrates repeatability, quantitative analyte recovery and minimal matrix suppression for the nine phthalate metabolites tested in urine.

Extraction of Aflatoxin M1 From Infant Formula Using ISOLUTE® Myco SPE Columns prior to LC-MS/MS Analysis

| Contributed by Biotage

This application note describes a Solid Phase Extraction (SPE) protocol for the extraction of aflatoxin M1 (AM1) internally standardized with aflatoxin B2 (AB2) from infant formula using ISOLUTE® Myco SPE columns with LC MS/MS.

Extraction of THC, THCA and Carboxy-THC from Oral Fluid

| Contributed by Biotage

This application note describes the extraction of THC, THCA and Carboxy-THC from oral fluid matrix collected using the Quantisall™ (Immunalysis) device, prior to GC/MS analysis.

Extraction of Thyroid Hormones, T3, rT3 and T4 from Serum

| Contributed by Biotage

This application note describes a polymer based-based strong anion exchange mixed-mode SPE protocol for the extraction of T3, rT3 and T4 from serum prior to LC-MS/MS analysis.

Analysis of THC and an Extended Metabolite Suite from Oral Fluid

| Contributed by Biotage

This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample loading volumes of either 300 μL or 800 μL.

Extraction of 1, α25-di-OH Vitamin D2, 1, α25-di-OH Vitamin D3, 25-OH Vitamin D2 and 25-OH Vitamin D3 from Serum Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis

| Contributed by Biotage

This application note describes a simple, clean and efficient Supported Liquid Extraction (SLE) protocol for the simultaneous extraction of 25-OH and 1, α25 di-OH-vitamin D2 and D3 metabolites from serum prior to LC-MS/MS analysis.

Extraction of Mycophenolic Acid (MPA) and Mycophenolic Acid Glucuronide (MPAG) from Serum Using ISOLUTE® SLE+ prior to LC-MS/MS

| Contributed by Biotage

This application note describes the extraction of the immunosuppressant drug mycophenolic acid and its metabolite mycophenolic acid glucuronide from fortified serum using ISOLUTE SLE+ in a 96-well plate format.

Fast Extraction of Basic Drugs from Urine with No Dry Down Step

| Contributed by Biotage

This application note describes the solid phase extraction of a variety of basic drugs in urine which are typically screened for forensic toxicology panels using EVOLUTE® EXPRESS CX in a 10 mg 96-well plate format.

Extraction of Phthalates in Commercial Milk Products

| Contributed by Biotage

This application note describes a novel sample cleanup strategy to address the complex matrix effects associated with commercial milk product variables while maintaining adequate analyte recovery and repeatability

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