Fields & Applications

Nanoporous gold helps ‘sieve out’ biological debris for fuss-free nucleic acid detection

Polyol-induced increases in thermal stability of antibodies. See how Differential scanning calorimetry can be used to assess the effects of polyol excipients on antibodies. DSC was used to monitor polyol-induced increases in thermal stability across seven monoclonal antibodies. The increases in thermal stability were measured as a function of polyol concentration and orientation of hydroxyl groups. It was found that increases in thermal stability were linear with respect to alcohol concentration.

In this report, we illustrate the utility of calorimetry in making better laboratory decisions. Examples will include improved protein construct selection for scale-up; direct measurement of the effects of mutations and post-translational modifications on protein stability; rapid optimization of solvent formulation; direct measurement of substrate and inhibitor binding affinity; determination of the mode of inhibitor binding; characterization of protein-protein interactions; and improved structural biology efficiency, when used in conjunction of other biophysical methods.

Contribution of variable domains to the stability of humanized IgG1 monoclonal antibodies Choosing the best antibody to progress in your biologic pipeline. Temperature-induced unfolding of three humanized IgG1 monoclonal antibodies and their Fab and Fc fragments was monitored by differential scanning calorimetry at neutral pH. With some exceptions, the thermogram of the intact antibody presents two peaks and the transition with the larger experimental enthalpy contains the contribution from the Fab fragments. Although the measured enthalpy was similar for all three Fab fragments studied, the apparent melting temperatures were found to vary significantly, even for Fab fragments originating from the same human germline.

See how you can use DSC to save you money in your contract development lab. This paper provides an overview of the workflow typically associated with preformulation projects at a contract development organization as well as provides a general framework for conducting preformulation studies that leverages the application of biophysical techniques such as DSC and traditional analytics by employing statistical design. A case study involving the formulation development of a monoclonal antibody is presented to detail the utility and potential limitations of DSC in support of preformulation for a variety of protein products.

The market for lactose-free products is growing rapidly and constantly and Europe is a worldwide leader in the lactose-free market.

Research into migration of packaging materials into food began decades ago. And despite the relatively inconspicuous nature of this niche area, it is characterized by some of the most significant modern-day challenges in food analysis.

Using saturation-transfer difference (STD) NMR and in situ NMR spectroscopic analyses of biocatalyzed reactions to optimize application of enzymes in organic synthesis.

Conducting an autopsy of the flaws of comparative bullet lead analysis in forensic practice

Exploring fluorescence spectroscopy’s ability to monitor quality – and reduce operating costs in wastewater treatment works