Technology

In this application note, a mixture of four proteins was characterized in a single SEC measurement using multiple detectors. The generally consistent response to the RI to proteins makes it a useful total protein concentration detector, while the UV detector can be used to measure the concentration of individual proteins when the extinction coefficient is known. This is particularly useful for proteins that elute in the solvent peak making RI measurements unreliable. Finally, light scattering measurements allow each peak to be identified by its molecular weight.

The ideal solvent for a material may not provide a sufficient refractive index difference for light scattering detection. This example of a biodegradable polymer shows how SELS is used to increase dn/dc to give enhanced detection sensitivity

In this application note, a purified polyclonal antibody (IgG) is separated using Size Exclusion Chromatography (SEC) and characterized using the Viscotek SEC-MALS 20, allowing calculation of molecular weight and radius of gyration (Rg).

Celebrating the Top 50 Women in analytical science. Nominations now open! 

App notes are undeniably useful, giving you access to expert knowledge in your field or helping you understand a new technique. But what if they went beyond simply providing static information? Enter AppsLab.

High-quality, automated sample preparation can help you take out the trash.

What’s going on in the analytical sciences?

How will modern genomics tools and other analytical breakthroughs drive forensic science?

A single snappy hashtag questions the very nature of scientific publishing

Two innovations that stood out from the crowd at Pittcon 2016