Technology

This application note describes the use of a Zetasizer Nano ZSP to characterize the charge state of spidroins in different formulations. The earliest stages of spidroin aggregation in different formulations are then assessed using low-temperature synamic light scattering (DLS) thermal trend experiments.

A sample of PMMA was analysed by Size Exclusion Chromatography with Triple detection, before and after sterilization. Reduction in the molecular weight was shown to be firstly by cleaving the polymer at the branching points.

In this application note, a selection of proteins are separated using Size Exclusion Chromatograpghy (SEC). The molecular weights of their oligomers and aggregates are measured by SEC-MALS and the results discussed.

Sitting Down With... Waseem Asghar, winner of the 2016 Humanity in Science Awards

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