Technology

Are you ready for the “unthinkable” – your laboratory computer systems suffering a titanic failure? Disaster recovery plans are crucial insurance against the unexpected.

The latest advances in the widely used technique of size exclusion chromatography (SEC) break new ground in terms of accuracy and sensitivity. This article assesses the information provided by multi-detector SEC and the potential benefits of recent innovations for polymeric food additive characterization.

When disruptive technology comes along, it can be hard to defend the technique under attack for some applications – even if we have embraced it for decades.

Finding a research partner just got a lot easier

Is a new and improved mass spectrometer the “Holy Grail” of chemical sensing?

The Morphologi G3-ID enables simultaneous physical and chemical characterization of particle samples, enabling outlier particles to be identified as being part of a product formulation or genuine foreign particle contaminants.

In this application note, a mixture of four proteins was characterized in a single SEC measurement using multiple detectors. The generally consistent response to the RI to proteins makes it a useful total protein concentration detector, while the UV detector can be used to measure the concentration of individual proteins when the extinction coefficient is known. This is particularly useful for proteins that elute in the solvent peak making RI measurements unreliable. Finally, light scattering measurements allow each peak to be identified by its molecular weight.

The ideal solvent for a material may not provide a sufficient refractive index difference for light scattering detection. This example of a biodegradable polymer shows how SELS is used to increase dn/dc to give enhanced detection sensitivity

In this application note, a purified polyclonal antibody (IgG) is separated using Size Exclusion Chromatography (SEC) and characterized using the Viscotek SEC-MALS 20, allowing calculation of molecular weight and radius of gyration (Rg).

Celebrating the Top 50 Women in analytical science. Nominations now open!