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Biotage

Application Notes by Biotage

Techniques & Tools Sample Preparation

Current Methodologies for Drugs of Abuse Urine Testing

| Contributed by Biotage

Examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.

Simultaneous Extraction of Aldosterone and Angiotensin I and II from Human Plasma Using EVOLUTE® EXPRESS ABN Prior to LC/MS Analysis

| Contributed by Biotage

This application note describes the simultaneous extraction of aldosterone and angiotensin from human plasma using the EVOLUTE® EXPRESS ABN Solid Phase Extraction plates prior to LC-MS/MS analysis.

Automated extraction of carboxy-THC from urine prior CG/MS analysis

| Contributed by Biotage

This application note describes the fully automated extraction of carboxy-THC from urine.

Quantisal Oral Fluid Collection Devices & SPE-CX Method Development – Practical Considerations

| Contributed by Biotage

The poster describes the relationship between 85 drugs of abuse (DOA) and their response to the recovery and matrix effects of Immunalysis’ Quantisal buffer as used with water as a surrogate oral fluid, synthetic oral fluid from UTAK, and the Quantisal device.

Evaluation of Matrix Component Removal Using a Novel Flow-through Scavenging Plate for Drugs of Abuse Testing in Urine

| Contributed by Biotage

This poster, presented at MSACL 2018 (P178) evaluates the extraction of a range of DOA from hydrolyzed and non-hydrolyzed urine using a novel flow-through matrix scavenging plate.

Comparison of Sample Preparation Options for the Extraction of a Panel of Endogenous Steroids from Serum Prior to UHPLC-MS/MS Analysis

| Contributed by Biotage

This poster compares sample preparation options for the extraction of a panel of endogenous steroids from serum. LC-MS/MS parameters were investigated for increased sensitivity.

Extraction of a Comprehensive Steroid Panel from Human Serum Using ISOLUTE® SLE+ Prior to LC/MS-MS Analysis

| Contributed by Biotage

This application note describes the extraction of a panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis.

Fields & Applications Forensics

Current Methodologies for Drugs of Abuse Urine Testing

| Contributed by Biotage

This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.

Fields & Applications Environmental

Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil Using ISOLUTE® EPH SPE Columns on Biotage® Extrahera™

| Contributed by Biotage

This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions.

Fields & Applications Forensics

An In-Well Hydrolysis Solid Phase Extraction Method Using EVOLUTE® HYDRO CX for 96 Licit and Illicit Drugs of Abuse from Urine Prior to UPLC-MS/MS

| Contributed by Biotage

This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates.

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