The Analytical Scientist Power List returns to celebrate the successes of the field’s leading lights!
08/03/2016 | Sponsored by Malvern Panalytical
This whitepaper reviews the methods available for measuring the key characteristics of polymers focusing on the benefits and value of gel permeation / size exclusion chromatography (GPC/SEC). Much of the paper talks exclusively about polymers, however many of the principles discussed are equally applicable to proteins or protein conjugate materials.
Traditionally in GPC, the sample dissolution solvent and the mobile phase are one and the same. However, a closer look at the demands of sample solvent and mobile phase suggests that this should not always be the case.
The complementarities of two techniques, dynamic light scattering (DLS) and static light scattering detector coupled with a size exclusion chromatography system (SEC-LS), are illustrated by studying a number of samples where a thermally denatured and aggregated protein sample were dosed at different levels into a non-denatured protein sample.
In this application note, intrinsic viscosity measurements were performed on the sRAGE protein to establish whether the detector was able to detect and characterize the conformational change induced by the presence of calcium. Light scattering molecular weight measurements were also performed. Data are also presented showing that the conformation of sRAGE changes in the presence and absence of calcium.
07/14/2016 | Stuart Adams
Then & Now, with Stuart Adams, Higher Analytical Chemist at Fera Science Ltd, York, UK
07/14/2016 | Sebastian Pantò
A new instrument could help with obtaining pure components from complex natural samples.
07/14/2016 | Rich Whitworth
Five experts share highlights from Riva 2016, the forum on microcolumn separations
06/17/2016 | Sponsored by Malvern Panalytical
This article explores how multiple detectors, particularly light scattering detectors, may be used for size-exclusion chromatography (SEC) in protein analysis. Using a light scattering detector as part of a multi-detector Size Exclusion Chromatography system provides the accurate molecular weight (MW) data needed to assess the activity, safety and clinical efficacy of therapeutic proteins.
The latest advances in the widely used technique of size exclusion chromatography (SEC) break new ground in terms of accuracy and sensitivity. This article assesses the information provided by multi-detector SEC and the potential benefits of recent innovations for polymeric food additive characterization.
06/16/2016 | David Elder
Detecting and controlling mutagenic impurities in pharmaceuticals is challenging. What is ICH M7 and what do you need in your analytical toolbox?
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