Gas Chromatography

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10 reasons for polymer characterization scientists. Gel-permeation chromatography (GPC) is an essential tool for the characterization of polymers. It allows polymer scientists to tailor a polymer’s properties to its end use requirements by controlling its molecular properties, since the two are inextricably linked.

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This whitepaper reviews the methods available for measuring the key characteristics of polymers focusing on the benefits and value of gel permeation / size exclusion chromatography (GPC/SEC). Much of the paper talks exclusively about polymers, however many of the principles discussed are equally applicable to proteins or protein conjugate materials.

Traditionally in GPC, the sample dissolution solvent and the mobile phase are one and the same. However, a closer look at the demands of sample solvent and mobile phase suggests that this should not always be the case.

The complementarities of two techniques, dynamic light scattering (DLS) and static light scattering detector coupled with a size exclusion chromatography system (SEC-LS), are illustrated by studying a number of samples where a thermally denatured and aggregated protein sample were dosed at different levels into a non-denatured protein sample.