Gas Chromatography

We speak with scientists using analytical techniques to solve some of the art world's mysteries

Products, partnerships, investments - what’s going on in the analytical science business world.

Hear from Kyle D’Silva as he discusses new developments in GC-MS technology for Extractables and Leachables testing

This whitepaper reviews the methods available for measuring the key characteristics of polymers focusing on the benefits and value of gel permeation / size exclusion chromatography (GPC/SEC). Much of the paper talks exclusively about polymers, however many of the principles discussed are equally applicable to proteins or protein conjugate materials.

Traditionally in GPC, the sample dissolution solvent and the mobile phase are one and the same. However, a closer look at the demands of sample solvent and mobile phase suggests that this should not always be the case.

The complementarities of two techniques, dynamic light scattering (DLS) and static light scattering detector coupled with a size exclusion chromatography system (SEC-LS), are illustrated by studying a number of samples where a thermally denatured and aggregated protein sample were dosed at different levels into a non-denatured protein sample.

In this application note, intrinsic viscosity measurements were performed on the sRAGE protein to establish whether the detector was able to detect and characterize the conformational change induced by the presence of calcium. Light scattering molecular weight measurements were also performed. Data are also presented showing that the conformation of sRAGE changes in the presence and absence of calcium.

Then & Now, with Stuart Adams, Higher Analytical Chemist at Fera Science Ltd, York, UK

A new instrument could help with obtaining pure components from complex natural samples.

Five experts share highlights from Riva 2016, the forum on microcolumn separations