Cookies

Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.
Techniques & Tools Mass Spectrometry, Proteomics, Technology

Accurate peptide retention data collection for retention modeling studies using the Q Exactive HF-X Orbitrap mass spectrometer

sponsored by Thermo Fisher

Introduction of proteomics provided separation scientists with virtually unlimited source of retention data: identification and assignment of retention properties of tens of thousands peptides in a few hours of instrument time has become a routine task. Nevertheless, accurate retention data collection for retention modeling or construction of comprehensive retention libraries for data independent acquisition protocols still represents a formidable task. This presentation provides step-by-step guide for construction of peptide retention library using 2D LC-MS/MS (high pH RP – low pH RP separation scheme, Q Exactive HF-X Orbitrap mass spectrometer) analysis of whole cell tryptic digests.

Learning Objectives of Webinar

  1. To introduce the concept of “chromatographic quality control” for accurate retention data collection via application of peptide retention standards;
  2. To learn about major parameters of separation system affecting separation selectivity, i.e. accuracy of retention data collection;
  3. To estimate expected output from a typical 2D LC-MS/MS acquisition using Q Exactive HF-X platform.

View on demand

Enjoy our FREE content!

Log in or register to gain full unlimited access to all content on the The Analytical Scientist site. It’s FREE and always will be!

Login

Or register now - it’s free and always will be!

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine
Register

Or Login via Social Media

By clicking on any of the above social media links, you are agreeing to our Privacy Notice.

Newsletter

Send me the latest from The Analytical Scientist.

Sign up now

Most Popular

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register