Genomics & DNA Analysis

A new DNA-based sensor shows promise for indirect detection of thallium

Nanoporous gold helps ‘sieve out’ biological debris for fuss-free nucleic acid detection

Contribution of variable domains to the stability of humanized IgG1 monoclonal antibodies Choosing the best antibody to progress in your biologic pipeline. Temperature-induced unfolding of three humanized IgG1 monoclonal antibodies and their Fab and Fc fragments was monitored by differential scanning calorimetry at neutral pH. With some exceptions, the thermogram of the intact antibody presents two peaks and the transition with the larger experimental enthalpy contains the contribution from the Fab fragments. Although the measured enthalpy was similar for all three Fab fragments studied, the apparent melting temperatures were found to vary significantly, even for Fab fragments originating from the same human germline.

Despite the recent bad press, cutting-edge analytical techniques are transforming the field of hair analysis

Lawrence (Larry) Lesko is a bioanalytical veteran with many years of academic and FDA experience. Now, as director of the University of Florida’s Center for Pharmacometrics and Systems Pharmacology, Larry uses metabolomics to discover biomarkers of drug toxicity.

Monika Dittman (Agilent Technologies) and Fabrice Gritti (Waters) offer a manufacturer point of view on the practical limits of LC.

The evidence contradicts those who say liquid chromatography has fully matured.

What drives someone to throw aside selfish pursuits to focus on projects with true philanthropic impact? Here, the winners of the 2015 Humanity in Science Award provide their answers.

Sitting Down With... Ruedi Aebersold, Deputy Head, Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Switzerland

Raman spectroscopy has evolved into a powerful characterization technique for many applications. What more needs to be done to make it effective in clinical analysis?