Data Analysis

The role that nanoparticles play in the rapidly developing field of ‘NanoMedicine’ has been discussed previously in Chapter 5. In this Chapter, we review the specific mechanisms by which such nanoparticles are designed and formulated and in which NTA has had a significant part to play.

The ability to quickly and reproducibly measure coffee ground particle size and size distributions is important during grinding operations to ensure that the coffee quality is maintained and that the correct grade is produced. The Mastersizer 3000 and the Funnel Sample Feeder for the Aero S dry powder disperser provides this capability, offering a rapid alternative to traditional sieve analysis.

The combination of static microscopy and Raman spectroscopy in the Morphologi G3-ID enables automated chemical identification of protein aggregates and other contaminants in a biotherapeutic sample, either in suspension via a thin-path wet cell or on a filter membrane.

The molecular weight, molecular weight distribution and size of three common polymers are measured using OMNISEC. This information can provide insight into the physical properties and behavior of these synthetic polymers.

Learn how Isothermal titration calorimetry can be used to guide protein (RNA)/ligand crystallization and to resolve common issues with crystallization of complexes.

Determining the components, and their proportion, in a protein sample is essential for the improvement of candidate formulations and characterization of final formulations.

GPC/SEC is mainly used for the characterization of macromolecules including synthetic and natural polymers (including polysaccharides) as well as proteins or RNA/DNA. This white paper provides an introduction to the technique in 30 minutes.

In this application note, we show that Malvern’s OmniSEC software can now communicate with Waters Empower® for the seamless exchange of sample sequences. This allows OmniSEC to follow the injections performed by a Waters SEC system and to synchronize data collection. A selection of proteins was used to demonstrate the measurement of protein molecular weight, oligomeric and aggregation state.

Four different polymers are analyzed by GPC/SEC to investigate the effect of molecular weight, dispersity and molecular structure on the final properties of the polymer.

Automated and reliable tools for spatially annotating metabolites from imaging mass spectrometry data are essential.