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Liquid Chromatography

Techniques & Tools Pharma & Biopharma

The Dose Makes the Poison!

| David Elder

Detecting and controlling mutagenic impurities in pharmaceuticals is challenging. What is ICH M7 and what do you need in your analytical toolbox?

Techniques & Tools Sample Preparation

Protein Applications for Advanced Multi-Detector Size-Exclusion Chromatography

| Sponsored by Malvern Panalytical

In this application note, a mixture of four proteins was characterized in a single SEC measurement using multiple detectors. The generally consistent response to the RI to proteins makes it a useful total protein concentration detector, while the UV detector can be used to measure the concentration of individual proteins when the extinction coefficient is known. This is particularly useful for proteins that elute in the solvent peak making RI measurements unreliable. Finally, light scattering measurements allow each peak to be identified by its molecular weight.

Techniques & Tools Sample Preparation

Solvent Enhanced Light Scattering Technique Enhances Gel Permeation Chromatography Analysis

| Chris Mirley, Sean Daughtry, Wei Sen Wong

The ideal solvent for a material may not provide a sufficient refractive index difference for light scattering detection. This example of a biodegradable polymer shows how SELS is used to increase dn/dc to give enhanced detection sensitivity

Techniques & Tools Sample Preparation

Measuring Antibody Molecular Weight by SEC-MALS

| Sponsored by Malvern Panalytical

In this application note, a purified polyclonal antibody (IgG) is separated using Size Exclusion Chromatography (SEC) and characterized using the Viscotek SEC-MALS 20, allowing calculation of molecular weight and radius of gyration (Rg).

Techniques & Tools Liquid Chromatography

Ion Legend

| Rich Whitworth

Sitting Down With... Chris Pohl, Vice President, Chromatography Chemistry, Thermo Fisher Scientific, San Francisco Bay Area, USA.

Techniques & Tools Gas Chromatography

Advancing Enantiomer Analysis

| Maurice Janssen

The story behind direct mass spectrometric detection of chiral molecules using MS-PECD – a winner of The Analytical Scientist Innovation Awards (TASIAs).

Techniques & Tools Mass Spectrometry

The Multidimensional Future of Proteomics

| John Yates, Shabaz Mohammed, Koen Sandra, Andrea Gargano

The proteome is practically infinite in its complexity. If we are ever going to fully unravel its secrets, the best separation tools – and the best know-how – must be combined. Here, four experts discuss the broad importance of proteomics, the potential of multidimensional liquid chromatography, and the challenges inherent in gaining insight beyond the “tip of the proteo-berg.”

Techniques & Tools Mass Spectrometry

LC-MS in the US

| Joanna Cummings

Are you hitting San Francisco? Or taking a San Antonio stroll? Here are our top picks for ASMS and HPLC 2016

Fields & Applications Liquid Chromatography

Guardians of Maximum Residue Limits

| Despo Louca, Magnus Jezussek, Sonja Masselter

Is pesticide residue analysis still a hot topic? Ahead of EPRW 2016 in Cyprus, current Chair – Despo Louca Christodoulou – and scientific organizing committee members Magnus Jezussek and Sonja Masselter discuss the importance of the field in challenging times.

Fields & Applications Liquid Chromatography

Determination of Lactose in Lactose-free Products Using HPAEC-PAD

| Mareike Margraf and Dr. Kate Monks

In many European countries, the limit of lactose in lactose-free labelled products has been lowered from 100 to 10 mg/100 g product in recent years (1).

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