Thin Layer Chromatography

The ideal solvent for a material may not provide a sufficient refractive index difference for light scattering detection. This example of a biodegradable polymer shows how SELS is used to increase dn/dc to give enhanced detection sensitivity

In this application note, a purified polyclonal antibody (IgG) is separated using Size Exclusion Chromatography (SEC) and characterized using the Viscotek SEC-MALS 20, allowing calculation of molecular weight and radius of gyration (Rg).

A sample of PMMA was analysed by Size Exclusion Chromatography with Triple detection, before and after sterilization. Reduction in the molecular weight was shown to be firstly by cleaving the polymer at the branching points.

This application note describes the analyses of a variety of aqueous (water-soluble) cellulose derivatives using the OMNISEC triple detection system and the subtle, yet distinct differences between these different derivatives that can be parsed out by the OMNISEC system

TLC-MS is here and still evolving – continual advances in coupling techniques and ambient mass spectrometry are giving rise to a renaissance. Here, we share the most promising techniques and open your eyes to the powerful – and flexible – combinations of TLC and MS.

For years, thin layer chromatography has been working hard in the background, the choice technique in certain applications. More recently, hyphenation with mass spectrometry has been a relatively quiet – but very real – game-changer. What have you been missing?

Many of the world’s leading experts came together for the 10th European Pesticide Residue Workshop (EPRW) in Dublin, Ireland, this summer for an international exchange of information and experience.

Sitting Down With… Salvatore Fanali, Head of the Capillary Electromigration and Chromatographic Methods Unit at the Institute of Chemical Methodologies, National Research Council (CNR), Monterotondo, Italy.

Deeper consideration of individual analytical unit processes offers a more systematic approach to method development, ensuring that we don’t forget the basics in an increasingly sophisticated world.