Standing Up for GC-MS – and Good Science
Though a recent paper about the data-distorting potential of gas chromatography in metabolomic studies is far from perfect, it does at least draw attention to the absolute criticality of robust experiment design – on both sides of the argument. And that’s got to be a good thing.
Karl Burgess |
Mingliang Fang’s paper in Analytical Chemistry, “Thermal Degradation of Small Molecules: A Global Metabolomic Investigation” (1), has been producing significant controversy since its appearance – and the paper’s conclusions are upsetting many people. Fang’s paper queries the entire concept of gas-chromatography mass spectrometry – a methodology used routinely in metabolomic investigations. By heating samples in a mimic of GC separation (GC generally uses a heat gradient to separate biomolecules) or derivitization followed by liquid chromatography, they observed significant changes in the metabolic profile observed – breakdown of triphosphates to monophosphates, for example.
Read the full article now
Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE and always will be!
Or register now - it’s free and always will be!
You will benefit from:
- Unlimited access to ALL articles
- News, interviews & opinions from leading industry experts
- Receive print (and PDF) copies of The Analytical Scientist magazine
Or Login via Social Media
By clicking on any of the above social media links, you are agreeing to our Privacy Notice.