Sample Preparation

Exploring the potential of microchip capillary electrophoresis to speed up sample throughput

Triple detection GPC/SEC combines measurements from multiple detectors to offer not only increased amounts of data, but also increased amounts of information, which is made available by the combination of different detectors (and unobtainable using individual detectors separately). The theory of triple detection GPC/SEC is described here.

This application note describes the analyses of a variety of aqueous (water-soluble) cellulose derivatives using the OMNISEC triple detection system and the subtle, yet distinct differences between these different derivatives that can be parsed out by the OMNISEC system

In this application note, molecular weight data from the Viscotek SEC-MALS 20 is combined with measurements of Rg and IV to study the structure of different polysaccharides including pullulan, dextran, hydroxypropyl cellulose (HPC), pectin and gum Arabic. The differences between conformation and Mark-Houwink plots are also studied.

The investigative power of multiple detectors. Here, we describe the analysis of a semi-purified protein mixture containing β-amylase. Step-by-step, we show how different properties of the proteins elicit a specific response in each detector and how coupling this data can be qualitatively and quantitatively evaluated.

Researchers use SPME and GC-MS to puncture claims that banned tire treatments are “undetectable”

Learn why isothermal titration calorimetry is a method of choice for accurate and precise determination of critical micellar concentration of surfactants for biochemical applications and find out how to analyse demicellization data from Isothermal Titration Calorimetry

In water analysis, direct injection of samples into your LC-MS/MS system (without sample preparation beyond filtration) is possible because of advances in instrumentation. However, matrix effects must be monitored carefully – and sometimes may even prove useful.

To formulate successfully with HA it is essential to understand the impact of factors such as molecular weight, molecular structure, concentration and degree of cross-linking on rheological characteristics such as viscoelasticity which are directly linked to aspects of product performance. Linking structural characteristics to product performance, via rheological properties, supports smart, fast, and effective formulation. The following study shows how rheology and particle size measurements can be used to characterize the physical properties of HA dermal fillers.

Polyol-induced increases in thermal stability of antibodies. See how Differential scanning calorimetry can be used to assess the effects of polyol excipients on antibodies. DSC was used to monitor polyol-induced increases in thermal stability across seven monoclonal antibodies. The increases in thermal stability were measured as a function of polyol concentration and orientation of hydroxyl groups. It was found that increases in thermal stability were linear with respect to alcohol concentration.