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Sample Preparation

Techniques & Tools Sample Preparation

Solvent Enhanced Light Scattering Technique Enhances Gel Permeation Chromatography Analysis

| Chris Mirley, Sean Daughtry, Wei Sen Wong

The ideal solvent for a material may not provide a sufficient refractive index difference for light scattering detection. This example of a biodegradable polymer shows how SELS is used to increase dn/dc to give enhanced detection sensitivity

Techniques & Tools Sample Preparation

Measuring Antibody Molecular Weight by SEC-MALS

| Sponsored by Malvern Panalytical

In this application note, a purified polyclonal antibody (IgG) is separated using Size Exclusion Chromatography (SEC) and characterized using the Viscotek SEC-MALS 20, allowing calculation of molecular weight and radius of gyration (Rg).

Techniques & Tools Technology

Garbage In, Garbage Out

| Guenter Boehm

High-quality, automated sample preparation can help you take out the trash.

Techniques & Tools Pharma & Biopharma

Language as Quality Control...

| Steven (Steve) Schultz

... or sonicate until the cockroaches disappear. When writing is central to the job of assuring health, safety, and quality, can you afford to be loose with language prone to misunderstanding? Why take a chance? Here are a few strategies to edit your written work for quality control.

Techniques & Tools Sample Preparation

Assessing the effect of formulation composition on spidroin charge and stability

| Sponsored by Malvern Panalytical

This application note describes the use of a Zetasizer Nano ZSP to characterize the charge state of spidroins in different formulations. The earliest stages of spidroin aggregation in different formulations are then assessed using low-temperature synamic light scattering (DLS) thermal trend experiments.

Techniques & Tools Sample Preparation

Case study: Effects of gamma-ray sterilization on high molecular weight

| Baljit Ghatora

A sample of PMMA was analysed by Size Exclusion Chromatography with Triple detection, before and after sterilization. Reduction in the molecular weight was shown to be firstly by cleaving the polymer at the branching points.

Techniques & Tools Sample Preparation

Viscotek SEC-MALS 20 to measure protein aggregation

| Sponsored by Malvern Panalytical

In this application note, a selection of proteins are separated using Size Exclusion Chromatograpghy (SEC). The molecular weights of their oligomers and aggregates are measured by SEC-MALS and the results discussed.

Techniques & Tools Proteomics

Don’t Neglect Protein QC

| Dieter Braun

Protein samples need – and deserve – special treatment, if you expect the best results from your experiments.

Techniques & Tools Capillary Electrophoresis

Microchip-CE: Smaller is Faster

| Friederike Winkhaus

Exploring the potential of microchip capillary electrophoresis to speed up sample throughput

Techniques & Tools Sample Preparation

Principles of Triple Detection GPC/SEC

| Sponsored by Malvern Panalytical

Triple detection GPC/SEC combines measurements from multiple detectors to offer not only increased amounts of data, but also increased amounts of information, which is made available by the combination of different detectors (and unobtainable using individual detectors separately). The theory of triple detection GPC/SEC is described here.

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