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Sample Preparation

Techniques & Tools Sample Preparation

Case study of a semi-pure β-amylase extract

| Sponsored by Malvern Panalytical

The investigative power of multiple detectors. Here, we describe the analysis of a semi-purified protein mixture containing β-amylase. Step-by-step, we show how different properties of the proteins elicit a specific response in each detector and how coupling this data can be qualitatively and quantitatively evaluated.

Techniques & Tools Mass Spectrometry

End of the Road

| James Strachan

Researchers use SPME and GC-MS to puncture claims that banned tire treatments are “undetectable”

Fields & Applications Sample Preparation

Analysis of demicellization data from isothermal titration calorimetry

Learn why isothermal titration calorimetry is a method of choice for accurate and precise determination of critical micellar concentration of surfactants for biochemical applications and find out how to analyse demicellization data from Isothermal Titration Calorimetry

Techniques & Tools Mass Spectrometry

The Pros and Cons of Direct Injection

| Torsten Schmidt

In water analysis, direct injection of samples into your LC-MS/MS system (without sample preparation beyond filtration) is possible because of advances in instrumentation. However, matrix effects must be monitored carefully – and sometimes may even prove useful.

Fields & Applications Sample Preparation

Evaluating the rheological properties of hyaluronic acid hydrogels for dermal filler applications

To formulate successfully with HA it is essential to understand the impact of factors such as molecular weight, molecular structure, concentration and degree of cross-linking on rheological characteristics such as viscoelasticity which are directly linked to aspects of product performance. Linking structural characteristics to product performance, via rheological properties, supports smart, fast, and effective formulation. The following study shows how rheology and particle size measurements can be used to characterize the physical properties of HA dermal fillers.

Fields & Applications Sample Preparation

Polyol-induced increases in thermal stability of antibodies by DSC

Polyol-induced increases in thermal stability of antibodies. See how Differential scanning calorimetry can be used to assess the effects of polyol excipients on antibodies. DSC was used to monitor polyol-induced increases in thermal stability across seven monoclonal antibodies. The increases in thermal stability were measured as a function of polyol concentration and orientation of hydroxyl groups. It was found that increases in thermal stability were linear with respect to alcohol concentration.

Fields & Applications Sample Preparation

The application of MicroCal VP Capillary DSC at a contract development organization

See how you can use DSC to save you money in your contract development lab. This paper provides an overview of the workflow typically associated with preformulation projects at a contract development organization as well as provides a general framework for conducting preformulation studies that leverages the application of biophysical techniques such as DSC and traditional analytics by employing statistical design. A case study involving the formulation development of a monoclonal antibody is presented to detail the utility and potential limitations of DSC in support of preformulation for a variety of protein products.

Fields & Applications Sample Preparation

Overview of Key Principles of Dynamic Light Scattering to protein therapeutic formulations – Part 4

This four-part series examines common issues and questions surrounding the principles, measurements and analysis of DLS data and discusses how to minimize the time required for and increase the accuracy of acquiring and interpreting DLS data during the biotherapeutic development process. In Part Four, we address frequently asked questions related to the application of DLS to the characterization of protein therapeutic formulations.

Fields & Applications Sample Preparation

Overview of Key Principles of Dynamic Light Scattering to protein therapeutic formulations – Part 3

This four-part series examines common issues and questions surrounding the principles, measurements and analysis of DLS data and discusses how to minimize the time required for and increase the accuracy of acquiring and interpreting DLS data during the biotherapeutic development process. In Part Three, we cover the basic types of DLS deconvolution algorithms used to extract the intensity weighted particle size distribution from the measured correlogram.

Fields & Applications Sample Preparation

Overview of Key Principles of Dynamic Light Scattering to protein therapeutic formulations – Part 2

This four-part series examines common issues and questions surrounding the principles, measurements and analysis of DLS data and discusses how to minimize the time required for and increase the accuracy of acquiring and interpreting DLS data during the biotherapeutic development process. In Part Two, we cover the influence of concentration effects and particle interactions on DLS results and provide a roadmap for identifying and distinguishing each type of concentration effect.

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