Pharma & Biopharma

In this application note we will look at two examples where the retention volume of a sample could not be used to obtain an accurate measure of the sample MW. We will also describe the additional information that can be obtained using these advanced detectors. This work has been performed in conjunction with the Institut Pasteur, Paris, France

The Zetasizer µV (MicroV) is a dual purpose light scattering instrument designed specifically for the analysis of proteins. Firstly, it is a highly sensitive cuvette based dynamic light scattering instrument with the same great performance as the market leading Zetasizer Nano. Secondly, by simply changing the cell, it becomes an absolute molecular weight and size detector that can be added to any Size Exclusion Chromatography system (such as the Viscotek TDAmax).

The Zetasizer Nano is the world's most widely used light scattering system, for measuring size, zeta potential and molecular weight. Applications range from characterizing high concentration colloids and nanoparticles, through to measurement of proteins and macromolecules in their native state, requiring as little as 12 microlitres of sample. The Zetasizer Nano can be used for the comprehensive characterization of proteins, colloids and nanoparticles, and is simple to use, highly sensitive, versatile and fast! This videos shows how quickly and easily a measurement can be made on the Zetasizer Nano.

Light scattering detectors play an important role in gel permeation chromatography and size exclusion chromatography (GPC/SEC) analysis because of their ability to directly measure molecular weight distribution. Multi angle light scattering (MALS) detectors are often chosen, in some instances because they provide the most accurate data for the measurement of the radius of gyration (Rg), and in others because they have become an accepted industry standard. The launch of Malvern Instruments’ new Viscotek SEC-MALS 20 detector extends commercial choice in this area and draws the technology into the spotlight.

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In recent years, the top ten pharmaceuticals sales list has been extensively populated with protein therapeutics, such as monoclonal antibodies and recombinant proteins, that are used to treat various life-threatening diseases, including cancer and autoimmune diseases.

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Without the rise of biopharmaceuticals, would separation science and mass spectrometry be where they are today?

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