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Data Analysis

Fields & Applications Sample Preparation

PEGylated proteins: Optimizing conjugation reactions using triple detection SEC

In this application note, one protein, interferon α-5, was conjugated with two different PEG molecules in different reactions.  The protein and PEG molecules were characterized individually using multi-detector SEC, followed by the PEGylated conjugates.  Their composition, molecular weights and intrinsic viscosities were measured. This work was performed by Celares GMBH, Germany with samples provided by DIGNA BIOTECH, Spain

Techniques & Tools Data Analysis

The Champions League of Measurement Science

| Peter Schoenmakers

A technique can only make it into the upper echelons of analytical chemistry with a strong, balanced team. And that means bringing together fundamental researchers, application specialists, and instrumentation experts on a well-supported playing field.

Techniques & Tools Sample Preparation

What You’ve Been Reading...

| Rich Whitworth

We dived into our analytics to find the most popular online articles. Here, we present a thought-provoking quote from each of the top ten.

Fields & Applications Data Analysis

Sharing Data – and Knowledge

| Steve Thomas

How to use a knowledge repository that doesn’t retire or leave you for a competitor.

Fields & Applications Sample Preparation

Application of Dynamic Light Scattering to Protein Therapeutic Formulations: Principles, Measurements and Analysis - 4. FAQs

Frequently asked questions related to the application of DLS to the characterization of protein therapeutic formulations

Techniques & Tools Sample Preparation

GPC Characterization of Nylon using Formic Acid for Reduced Cost per Analysis

| Sponsored by Malvern Panalytical

GPC/SEC can be used to characterize commodity or bulk manufactured polymers. Here we describe how the use of formic acid instead of hexafluoroisopropanol (HFIP) as the dissolution solvent and/or mobile phase can lead to improvements in both cost per analysis and health and safety. 

Fields & Applications Technology

Characterization of Protein-Protein Interactions using triple detection size exclusion chromatography (SEC-TD)

Work performed in conjunction with the Institut Pasteur, Paris, France.In this application note, triple detection size exclusion chromatography (SEC-TD) is used to understand the ability of proteins to form ordered, structured complexes. The controlling mechanism behind these protein complexes is fundamental to our knowledge of protein signaling pathways and the physiological responses they control.

Techniques & Tools Data Analysis

Why We Need the Theory of Sampling

| Kim H. Esbensen, Claas Wagner

Without representative sampling, measurement uncertainty is compromised. Here, we present the current Theory of Sampling versus Measurement Uncertainty debate.

Fields & Applications Sample Preparation

Transforming a black art: adding rheometry to the formulation toolkit

Rheology is the science of material flow. It is also a science with a reputation for some fairly daunting mathematics. But look past these first principles and rheology has immediate relevance in formulation which is readily appreciated and easily exploited.

Techniques & Tools Data Analysis

Chemometric Magic

| Ewa Szymańska

More than a year has passed since I expressed my hopes for chemometrics to take center stage in this magazine. Where are we now?

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