Data Analysis

In this application note, we show that Malvern’s OmniSEC software can now communicate with Waters Empower® for the seamless exchange of sample sequences. A selection of proteins was used to demonstrate the measurement of protein molecular weight, oligomeric and aggregation state.

In this application note, one protein, interferon α-5, was conjugated with two different PEG molecules in different reactions.  The protein and PEG molecules were characterized individually using multi-detector SEC, followed by the PEGylated conjugates.  Their composition, molecular weights and intrinsic viscosities were measured. This work was performed by Celares GMBH, Germany with samples provided by DIGNA BIOTECH, Spain

A technique can only make it into the upper echelons of analytical chemistry with a strong, balanced team. And that means bringing together fundamental researchers, application specialists, and instrumentation experts on a well-supported playing field.

We dived into our analytics to find the most popular online articles. Here, we present a thought-provoking quote from each of the top ten.

How to use a knowledge repository that doesn’t retire or leave you for a competitor.

Frequently asked questions related to the application of DLS to the characterization of protein therapeutic formulations

GPC/SEC can be used to characterize commodity or bulk manufactured polymers. Here we describe how the use of formic acid instead of hexafluoroisopropanol (HFIP) as the dissolution solvent and/or mobile phase can lead to improvements in both cost per analysis and health and safety. 

Work performed in conjunction with the Institut Pasteur, Paris, France.In this application note, triple detection size exclusion chromatography (SEC-TD) is used to understand the ability of proteins to form ordered, structured complexes. The controlling mechanism behind these protein complexes is fundamental to our knowledge of protein signaling pathways and the physiological responses they control.

Without representative sampling, measurement uncertainty is compromised. Here, we present the current Theory of Sampling versus Measurement Uncertainty debate.

Rheology is the science of material flow. It is also a science with a reputation for some fairly daunting mathematics. But look past these first principles and rheology has immediate relevance in formulation which is readily appreciated and easily exploited.