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Preparative/Process Chromatography

Business & Education Sample Preparation

Detection and quantification of large protein aggregates

| Sponsored by Malvern Panalytical

The complementarities of two techniques, dynamic light scattering (DLS) and static light scattering detector coupled with a size exclusion chromatography system (SEC-LS), are illustrated by studying a number of samples where a thermally denatured and aggregated protein sample were dosed at different levels into a non-denatured protein sample.

Business & Education Sample Preparation

Intrinsic viscosity measurements on sRAGE protein

| Sponsored by Malvern Panalytical

In this application note, intrinsic viscosity measurements were performed on the sRAGE protein to establish whether the detector was able to detect and characterize the conformational change induced by the presence of calcium. Light scattering molecular weight measurements were also performed. Data are also presented showing that the conformation of sRAGE changes in the presence and absence of calcium.

Techniques & Tools Sample Preparation

The Benefits of Multi-Detector SEC for Protein Analysis'

| Sponsored by Malvern Panalytical

This article explores how multiple detectors, particularly light scattering detectors, may be used for size-exclusion chromatography (SEC) in protein analysis. Using a light scattering detector as part of a multi-detector Size Exclusion Chromatography system provides the accurate molecular weight (MW) data needed to assess the activity, safety and clinical efficacy of therapeutic proteins.

Techniques & Tools Sample Preparation

Optimizing Food Additive Choice: Exploring the Value of Recent Advances in SEC Technology

| Sponsored by Malvern Panalytical

The latest advances in the widely used technique of size exclusion chromatography (SEC) break new ground in terms of accuracy and sensitivity. This article assesses the information provided by multi-detector SEC and the potential benefits of recent innovations for polymeric food additive characterization.

Techniques & Tools Sample Preparation

Protein Applications for Advanced Multi-Detector Size-Exclusion Chromatography

| Sponsored by Malvern Panalytical

In this application note, a mixture of four proteins was characterized in a single SEC measurement using multiple detectors. The generally consistent response to the RI to proteins makes it a useful total protein concentration detector, while the UV detector can be used to measure the concentration of individual proteins when the extinction coefficient is known. This is particularly useful for proteins that elute in the solvent peak making RI measurements unreliable. Finally, light scattering measurements allow each peak to be identified by its molecular weight.

Techniques & Tools Sample Preparation

Measuring Antibody Molecular Weight by SEC-MALS

| Sponsored by Malvern Panalytical

In this application note, a purified polyclonal antibody (IgG) is separated using Size Exclusion Chromatography (SEC) and characterized using the Viscotek SEC-MALS 20, allowing calculation of molecular weight and radius of gyration (Rg).

Techniques & Tools Sample Preparation

Case study: Effects of gamma-ray sterilization on high molecular weight

| Baljit Ghatora

A sample of PMMA was analysed by Size Exclusion Chromatography with Triple detection, before and after sterilization. Reduction in the molecular weight was shown to be firstly by cleaving the polymer at the branching points.

Techniques & Tools Sample Preparation

Viscotek SEC-MALS 20 to measure protein aggregation

| Sponsored by Malvern Panalytical

In this application note, a selection of proteins are separated using Size Exclusion Chromatograpghy (SEC). The molecular weights of their oligomers and aggregates are measured by SEC-MALS and the results discussed.

Techniques & Tools Sample Preparation

Principles of Triple Detection GPC/SEC

| Sponsored by Malvern Panalytical

Triple detection GPC/SEC combines measurements from multiple detectors to offer not only increased amounts of data, but also increased amounts of information, which is made available by the combination of different detectors (and unobtainable using individual detectors separately). The theory of triple detection GPC/SEC is described here.

Techniques & Tools Sample Preparation

Using OMNISEC to Understand the Effect of Molecular Weight and Structure on the Performance Characteristics of Cellulose Derivatives

| Sponsored by Malvern Panalytical

This application note describes the analyses of a variety of aqueous (water-soluble) cellulose derivatives using the OMNISEC triple detection system and the subtle, yet distinct differences between these different derivatives that can be parsed out by the OMNISEC system

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