Sample Preparation

Whey is a by-product of the cheese-making process, and Whey Protein Isolate (WPI) is a dietary supplement and food ingredient created by separating components from milk. It contains a high percentage of pure protein and can be pure enough to be virtually lactose free, carbohydrate free, fat free, and cholesterol free. In this application note, a sample of WPI was characterized using the Viscotek TDAmax. The 4 main components in this mixture were separated and individually characterized.

This application note presents an example of how calorimetry aided the formulation development for ProX by providing insight into excipient-protein interactions. Polysorbate-80 and phenol are examined here as potential additives to the formulation buffer for ProX.

Analytical QbD (AQbD) promises to extend the rigor and benefits of QbD into the area of analytical method development.

Many sample preparation techniques originated a long, long time ago. Should we be satisfied with old separation magic or does our whole approach need to be re-cast?

New components and measurement techniques continue to be introduced for the Zetasizer Nano. New to the system are a Surface Zeta Potential accessory and a new diffusion barrier method for improved zeta potential measurements of protein samples. Why is the Malvern Zetasizer Nano so popular and found in a broad range of applied and fundamental scientifi c projects across all industries and fields? Because, it is simply the most dynamic and broad ranged instrument on the market for solving technical issues in today’s research environment and it continues to evolve with new capabilities. Here are 10 reasons why you should choose the Zetasizer Nano:

The Zetasizer NanoSampler is a sample delivery system that ensures highly precise and reproducible automated loading of samples into your Zetasizer Nano for particle size measurements. The NanoSampler adds automation and unattended operation in a versatile, compact package, maximising the productivity of your Zetasizer Nano.

This application note provides an example of how Archimedes can be used to detect and quantify the formation of protein sub-visible particles and the introduction of silicone oil droplets, in response to shear stress.

In this application note we will look at two examples where the retention volume of a sample could not be used to obtain an accurate measure of the sample MW. We will also describe the additional information that can be obtained using these advanced detectors. This work has been performed in conjunction with the Institut Pasteur, Paris, France

Polymers can be loosely grouped into two structural categories – linear and branched.

Work performed in conjunction with the Membrane Protein Laboratory, Imperial College, London