Cookies

Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.

Data Analysis

Business & Education Sample Preparation

Detection and quantification of large protein aggregates

| Sponsored by Malvern Panalytical

The complementarities of two techniques, dynamic light scattering (DLS) and static light scattering detector coupled with a size exclusion chromatography system (SEC-LS), are illustrated by studying a number of samples where a thermally denatured and aggregated protein sample were dosed at different levels into a non-denatured protein sample.

Business & Education Sample Preparation

Intrinsic viscosity measurements on sRAGE protein

| Sponsored by Malvern Panalytical

In this application note, intrinsic viscosity measurements were performed on the sRAGE protein to establish whether the detector was able to detect and characterize the conformational change induced by the presence of calcium. Light scattering molecular weight measurements were also performed. Data are also presented showing that the conformation of sRAGE changes in the presence and absence of calcium.

Techniques & Tools Clinical

Addressing Diagnostic Error

| Greg Miller

How can we harmonize lab testing and pathology consultations to prevent lab-related diagnostic errors?

Techniques & Tools Data Analysis

What We Learnt: Riva 2016

| Rich Whitworth

Five experts share highlights from Riva 2016, the forum on microcolumn separations

Techniques & Tools Sample Preparation

The Benefits of Multi-Detector SEC for Protein Analysis'

| Sponsored by Malvern Panalytical

This article explores how multiple detectors, particularly light scattering detectors, may be used for size-exclusion chromatography (SEC) in protein analysis. Using a light scattering detector as part of a multi-detector Size Exclusion Chromatography system provides the accurate molecular weight (MW) data needed to assess the activity, safety and clinical efficacy of therapeutic proteins.

Fields & Applications Sample Preparation

Sizing up the Solution

This article explores the role of different components within drilling muds, including barite and calcium carbonate. The need to achieve optimum particle sizes is examined, the application of laser diffraction is considered within this context. Fast, efficient and having a wide measurement range, laser diffraction delivers accurate, precise and reproducible particle size information. Offering automated analysis, it is a preferred method for drilling mud particle sizing and the focus of ongoing API efforts to develop a standard in this area.

Fields & Applications Sample Preparation

Ten Ways to... Control Rheology by Changing Particle Properties

The newest addition to the popular ‘10 ways to…’ series of educational papers provides practical guidance on developing high performance suspension products with enhanced consumer appeal. ‘10 ways to...control rheology by changing particle properties’ explores how controlling particle characteristics, such as particle size distribution, concentration and zeta potential, enables formulators to engineer suspension performance. Suspensions are used in the formulation of a wide range of every day products from medicines, drinks and foodstuffs to paints, inks and coatings. This paper is therefore valuable to formulators working across a wide range of industries.

Techniques & Tools Sample Preparation

Optimizing Food Additive Choice: Exploring the Value of Recent Advances in SEC Technology

| Sponsored by Malvern Panalytical

The latest advances in the widely used technique of size exclusion chromatography (SEC) break new ground in terms of accuracy and sensitivity. This article assesses the information provided by multi-detector SEC and the potential benefits of recent innovations for polymeric food additive characterization.

Fields & Applications Sample Preparation

Identification of foreign particulate matter using the Morphologi G3-ID

The Morphologi G3-ID enables simultaneous physical and chemical characterization of particle samples, enabling outlier particles to be identified as being part of a product formulation or genuine foreign particle contaminants.

Techniques & Tools Sample Preparation

Protein Applications for Advanced Multi-Detector Size-Exclusion Chromatography

| Sponsored by Malvern Panalytical

In this application note, a mixture of four proteins was characterized in a single SEC measurement using multiple detectors. The generally consistent response to the RI to proteins makes it a useful total protein concentration detector, while the UV detector can be used to measure the concentration of individual proteins when the extinction coefficient is known. This is particularly useful for proteins that elute in the solvent peak making RI measurements unreliable. Finally, light scattering measurements allow each peak to be identified by its molecular weight.

Newsletter

Send me the latest from The Analytical Scientist.

Sign up now

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register